Immagine di CHIARA RAMPAZZO

CHIARA RAMPAZZO

Title: Professore associato confermato

SSD: BIO/06 - Comparative Anatomy and Citology

Address: VIALE GIUSEPPE COLOMBO, 3 - VIA UGO BASSI, 58/B - PADOVA

Phone: 0498276456

E-mail: chiara.rampazzo.1@unipd.it

Teaching in current academic year

Course Degree
MOLECULAR AND CELLULAR BIOLOGYIndustrial Biotechnology
LARGE-SCALE CELL CULTURES AND BIOMOLECULES PRODUCTIONIndustrial Biotechnology
CELL BIOLOGYMolecular Biology

Curriculum

Affiliation: Dept. of Biology Position: Associate Professor at the Department of Biology of the University of Padova, Italy Education and academic career: 1994: Laurea in Biological Sciences, University of Padova 1995: Erasmus Fellowship, Department of Genetics, University of Stockholm, Sweden 1996-1998: PhD student at the Department of Biology, University of Padova 1997: EMBO short-term Fellowship, Department of Biochemistry, Karolinska Institute, Stockholm, Sweden 1998: EMBO short-term Fellowship, Department of Biochemistry, Karolinska Institute, Stockholm, Sweden 1999-2003: Postdoctoral fellow, Department of Biology, University of Padova 2004-2017: Assistant Professor in Cell Biology, Department of Biology, University of Padova 2017-present: Associate Professor in Cell Biology, Department of Biology, University of Padova Co-author of 30 peer-reviewed papers Total citation: 956 H factor: 18

Articles published in the last 5 years

  • Enhanced Silkworm Cecropin B Antimicrobial Activity against Pseudomonas aeruginosa from Single Amino Acid Variation
    ACS INFECT DIS
    2019
    Romoli O, Mukherjee S, Mohed SA, Dutta A, Montali A, Franzolin E, Brady D, Zito F, Bergantino E, Rampazzo C, Testamenti G, Bhutia A, Sandrelli F
  • dNTP metabolism links mechanical cues and YAP/TAZ to cell growth and oncogene-induced senescence
    EMBO J
    2018
    Santinon G, Brian I, Pocaterra A, Romani P, Franzolin E, Rampazzo C, Bicciato S, Dupont S
  • A defective dNTP pool hinders DNA replication in cell cycle-reactivated terminally differentiated muscle cells
    CELL DEATH DIFFER
    2017
    Pajalunga D, Franzolin E, Stevanoni M, Zribi S, Passaro N, Gurtner A, Donsante S, Loffredo D, Losanno L, Bianchi V, Russo A, Rampazzo C, Crescenzi M
  • The druggability of intracellular nucleotide-degrading enzymes
    CANCER CHEMOTH PHARM
    2016
    Rampazzo C, Tozzi MG, Dumontet C, Jordheim LP
  • The dNTP triphosphohydrolase activity of SAMHD1 contributes to the mitochondrial DNA depletion associated with genetic deficiency of deoxyguanosine kinase
    J BIOL CHEM
    2015
    Franzolin E, Salata C, Bianchi V, Rampazzo C




Research area

Regulation of DNA precursors in mammalian cells during proliferation and quiescence: implications for the stability of the nuclear and mitochondrial genomes in health and disease. Disturbances of the relative concentrations and the dynamics of DNA precursor (dNTP) pools affect the accuracy and efficiency of DNA synthesis. Main effects are increased frequency of mutations, structural alterations of chromosomes, destabilization or depletion of the mitochondrial genome. We study the mechanisms that keep dNTP pools in tune with the needs of nuclear and mitochondrial DNA replication and repair. The enzyme network includes both synthetic and catabolic enzymes and we analyze their functional interactions in cultured mammalian cells.

Proposals for thesis

Relationships between telomere metabolism and dNTP pool sizes. The aim of the project is to examine the effect of dNTP imbalance on telomere length by quantitative fluorescence in situ hybridization (Q-FISH) and quantitative PCR. Deoxynucleotide imbalance and mtDNA stability in mammalian cells. The aim of the project is to understand cellular mechanisms for the maintenance of mtDNA integrity and copy number by Next Generation Sequencing and quantitative PCR.