Location: Vallisneri's building, Viale G. Colombo n. 3 / via Ugo Bassi n. 58/B

Laboratory: 5th floor south, rooms n. 14, 15, 27.

Opening hours: From Monday to Friday 8:00 a.m. to 6:00 p.m.

Facility manager: Prof. Graziano Martello; Prof. Milena Bellin

Technical manager: Dr. Evgenii Lysenko

Contacts:

• E-mail:  ipsc.facility.biologia@unipd.it

• Telephones: +39 0498279394 - +393894480244

Overview

iPSCs Facility is located on the 5th floor, South Wing of the Vallisneri Building, rooms 14, 15 and 27.

(GEOTEC CODE: U.0048-06-044/047/048/074)

The facility consists of five separate rooms. Room 1 is equipped with one laminar flow hood, one CO2/N2 incubator, one centrifuge, one refrigerator (+4 and -20 C°), and one water bath. Room 2 is equipped with one laminar flow hood with two working stations. Room 3 is equipped with one CO2 incubator. Room 4 is equipped with a Nikon inverted fluorescence microscope, a laminar flow hood and room 5 is equipped with a liquid nitrogen tank and refrigerator (+4 and -20 C°).

The hoods and the incubators are exclusively dedicated to work with cultures of pluripotent stem cells (maintenance and differentiation). The microscope is equipped with a camera, and three fluorescence filters (560/470/285 LED units). It can be used by all users for routine monitoring of iPSC cultures, furthermore it will be possible to book longer slots for fluorescence acquisition. Liquid nitrogen tank is provided for storage of frozen cells. Two refrigerators are for the storage of medium, supplements, citokines and other reagents needed for the work with iPSCs.

Guidelines

Access to the facility is possible only with the authorization of the facility manager and after training from the facility technician.

Prior to their use in the facility, all PSC/iPSC lines must be tested for mycoplasma. The facility can be used for any type of PSC cells differentiation and maintenance of their derivatives.

New users must be specifically trained before working in the facility.

The specific training includes the study of the documentation provided by the Facility on the

handling of the equipment, safety rules and general rules of maintaining the culture of PSCs.

These training sessions are held by appointment. The candidate user should contact the Facility technician by e-mail (evgenii.lysenko@unipd.it) for documentation and the appointment.

Users cannot work in the iPSCs Facility unless all the training requirements have been met.

Access to the Facility is restricted to those people whose presence is required for experimental or support purposes. The Facility managers and technicians have the final responsibility of assessing each circumstance and deciding who may enter or work in the laboratory.

The room is accessible from Monday to Friday 8:00 a.m. to 6:00 p.m. If the researcher needs to access the laboratory at different hours or on holidays, Facility manager must be informed.

The access to the Facility is subject to reservation using the booking system at

https://service.biologia.unipd.it/gestione_dipartimento/login with username and password. This is essential for the safety of all users. Moreover, the logbook will be used for the calculation of the expenses.

It is mandatory that all personnel wear PPE (personal protective equipment) i.e. lab coats and gloves. Lab coats will be sent for cleaning every 3 weeks. All personnel must wash their hands after removing gloves. Eating, drinking, and  storing food is not allowed.

Clean and sanitize with 70% ethanol anything that has been touched before leaving the room.

Equipment

n. 1 Fume hoods MSC Advantage 1.2 (Thermo Scientific)

n. 1 Fume hood Gelare Flow Laboratories TC72

n. 2 -20/+4°C Refrigerators Whirlpool

n. 1 Water Bath.

n. 1 Bench centrifuge Eppendorf™5805000327.

n. 1 Series 8000 DH CO2 Incubator (Thermo Scientific)

n. 1 CO2/N2 Incubator CB 170 BINDER with O2 0.2-20% regulation.

n. 1 NIKON Inverted Fluorescence Microscope TS2-FL (560/470/385 LED units).