
Plant Genome Editing and Phenotyping
Location
Vallisneri’s building, Viale G. Colombo n. 3 / via Ugo Bassi n. 58/b
Office / Laboratories: 4a, 4th South floor
Phone: 049 8276265
Contacts
alessandro.alboresi@unipd.it
alessandra.bellan@unipd.it
pge.biologia@unipd.it
Opening hours
Monday to Friday: from 09:00 to 17:00
Overview
Plant biology is experiencing a revolution with genome editing technologies that allow fast and efficient genetic changes. PGE is born to support the genome editing experiments in terms of
- Experiment design
- Cell culture and Transformation
- Transformant isolation and Genotyping
- DNA extraction from different plant samples
- Plant cultivation
- Phenotyping
- Maintenance of collections of lines / plants / material.
Facility Manager
Prof. Alessandro Alboresi
Staff
Full time: Alessandra Bellan
Part Time: Elisabetta Barizza, Luca Cesarotto, Stefania Marcato, Anna Pavanello, Anna Segalla
Instruments:
- Cell culture and plant cultivation: 9 growth chambers with different illumination / temperature / humidity and light regime.
- Transformation: particle gun from Beambio and electroporator from ECM® 630 Electroporator;
- Phenotyping: Fluorcam from Photon System Instruments (closed and open), stereomicroscope with camera;
- LI-6800 Photosynthesis System for measurement of Leaf-Level Gas Exchange & Fluorescence
- Felix F-750 for Near Infrared Spectroscopy and measurement of fruit quality.
Activities:
- Design of genome editing approaches (eg. support in guide RNA design)
- Optimization of DNA extraction protocols from plant samples (also critical ones, rich of phenols etc)
- Training to use the instruments available for transformation and phenotyping.
- Management and maintenance of the growth chambers entrusted to the facility.
- Experimental Greenhouse Management.
- Plant growing support.
- Maintenance of solid plant cell cultures, lines of plants, collecting seeds ex Arabidopsis.
- Maintenance of algae / cyanobacteria strains collection.
- Measurement of leaf gas exchange.
- Measurement of fruit quality.