COST JUSTIFICATION

1) Tank maintenance
Daily feeding and cleaning; periodic water parameter and health status monitoring.
Personnel: 1 Full professor 2 hrs/year, 1 Associate professor 4 hrs/year, 1 Post-doc 48 hrs/year
Overheads 18%

2) Zebrafish lines
Fish line crossing, screening, genotyping and shipping. Microscopy observations and instruments depreciation. In vitro fertilization and embryo raising.
Personnel: 1 Full professor 2 hrs/year, 1 Associate professor 4 hrs/year, 1 Post-doc 48 hrs/year
Overheads 18%

3) Zebrafish genotyping
Technical procedures, plasticware and reagents.
Personnel: 1 Post-doc 36 hrs/year
Overheads 18%

4) Cryopreservation of fish sperm or testis
Technical procedures, sperm quality check, plasticware, glassware, cryopreservation solutions, liquid nitrogen.
Personnel: 1 Full professor 0.5 hrs/session, 1 Associate professor 2 hrs/session, 1 Post-doc 4 hrs/session
Overheads 18%

5) Cell transplantation
Fish line crossing, cell microinjection, follow-up (embryo raising, monitoring and imaging).
Personnel: 1 Associate professor 9 hrs/session
Overheads 18%

6) Zebrafish courses

Course "Zebrafish handling" (12 hrs, 3 days)
Materials and instrumentation.
Personnel: 1 Full professor 1 hr, 1 Associate professor 1 hr, 1 Post-doc 15 hrs
Overheads 18%

Course "Zebrafish genotyping" (12 hrs, 3 days)
Materials and instrumentation.
Personnel: 1 Full professor 1 hr, 1 Associate professor 1 hr, 1 Post-doc 20 hrs
Overheads 18%

Course "Zebrafish microinjection" (20 hrs, 5 days)
Materials and instrumentation.
Personnel: 1 Full professor 1 hr, 1 Associate professor 1 hr, 1 Post-doc 30 hrs
Overheads 18%

Course "Zebrafish genome editing" (20 hrs, 5 days)
Materials and instrumentation.
Personnel: 1 Full professor 1 hr, 1 Associate professor 1 hr, 1 Post-doc 35 hrs
Overheads 18%

Prices are tax-free and intended for external users. For internal users, Facility regulations are applied.