HiTS@UniPD costs (in Euro)
|Hourly Operetta with automation||50.00||75.00||100.00||150.00||68.00||73.00||75.00||73.00|
|Genome-Wide Library Preparation||6100.00||9150.00||12200.00||18300.00||8130.00||8700.00||9150.00||8700.00|
|Genome-Wide Screen Acquisition||Ask||Ask||Ask||Ask||Ask||Ask||Ask||Ask|
|Myriascreen Library Preparation||6000.00||9000.00||12000.00||18000.00||8000.00||8500.00||9000.00||8500.00|
|Myriascreen 2 Confocal Library Data Acquisition||Ask||Ask||Ask||Ask||Ask||Ask||Ask||Ask|
|Hourly Columbus Workstation||3.00||4.50||6.00||9.00||4.00||4.20||4.50||4.20|
|Hourly Harmony Workstation||6.00||9.00||12.00||18.00||7.50||8.40||9.00||8.40|
|Hood precision Liquid Handler||5.00||7.50||10.00||15.00||6.80||7.30||7.50||7.30|
|QIAcube Instrument *per sample||1.50||4.00||6.00||10.00||3.30||3.80||4.00||3.80|
Costs may slightly differ due to reagents market price variation.
Why are the fees different for users with different affiliations?
There are 4 main fees categories: Dept. of Biology (DiBio), all other Depts of the University of Padua (UniPD), Other Academia and non profit organizations (Academia) and Industry. DiBio supports the facility with dedicated technical personnel and with its own budget. Discounts are applied on the UniPD fee for the members of the User Community identified in the Infrastructure Grant of UniPD that supported the setup of the Facility. Discounts are based on the contribution to the total cost of the facility (442555€, excluding Operetta, Precision XS, Myriascreen library that were all donated by L. Scorrano). VIMM contributed with 40000€ (in kind, donating the Quiacube and its reagents), DSF and DMM with 10000€, DSB supported the project but did not contribute financially to it. Discounts are therefore approximately 9% for VIMM and 2.25% for DSF and DMM.
What do the fees include?
The hourly rates include only the use of the Operetta High throughput widefield/confocal microscope, or of the Operetta with the automated platform for plate handling. A technician from the facility will assist the operator in setting up the experiment.
The Genome wide cost includes: Operetta, Automation, Human or Mouse Genome Wide library. This cost refers to 1 replica. A facility technician will assist the operator in setting up the experiment.
The Myriascreen cost includes: Operetta, Automation, Myriascreen (Sigma) small molecule library. This cost refers to 1 replica.
Plates, transfection reagents, tips, cell culture reagents and controls (scr siRNA or other meaningful reference conditions like solvent, untreated samples etc.) must be provided by the user. Make and model of tips are defined by the HiTS@UniPD facility personnel. Plates are also defined by the HiTS@UniPD facility personnel. In case of special needs, other plate types can be tested with the facility and their use implemented. Costs for testing plates will be charged to the user based on the hourly rates above. Before starting a genome wide or a Myriascreen screening, the users must have all the conditions optimized by running preliminary tests to verify that their readout is robust enough. They must also run preliminary tests to verify acquisition times and plate position effects on their readout. These tests will be charged the hourly rate.
The Columbus Workstation fee includes the use of a workstation on the 6th floor. The workstation is connected to the Columbus Server with a multiplatform image analysis system that allows you to analyse data from a variety of imaging software, including of course the Harmony Software of the Operetta. It does not include the storage of your images, analyses and summary data that must be done on an external hard drive (we suggest storage units of>500GB)
What if I want to run a targeted screening (like a kinome screening, or a FDA-approved compounds screening)?
If your screening is targeted, we negotiated special discounts for smaller siRNA libraries that must be purchased by the user. To use the facility, you will be charged the Hourly Operetta with automation fee. Consider that for a screening of the kinome (delivered in 384 well format) we foresee that you will need 4 hrs in widefield (3 channels, 6 field per well) or 16 hrs in confocal (2 channels, 2 fields per well) mode. To screen a FDA approved library delivered in 384 well format, you will need for the same conditions 8 hrs in widefield and 32 hrs in confocal.