Location
Vallisneri’s building, Viale G. Colombo n. 3 / via Ugo Bassi n. 58/b
Office / Laboratories: 4a, 4th South floor
Phone: 049 8276265

Contacts
alessandro.alboresi@unipd.it
alessandra.bellan@unipd.it
pge.biologia@unipd.it

Opening hours
Monday to Friday: from 09:00 to 17:00

Overview
Plant biology is experiencing a revolution with genome editing technologies that allow fast and efficient genetic changes. PGE is born to support the genome editing experiments in terms of

1.  Experiment design
2.  Cell culture and Transformation
3.  Transformant isolation and Genotyping
4.  DNA extraction from different plant samples
5.  Plant cultivation
6.  Phenotyping
7.  Maintenance of collections of lines / plants / material.

Facility Manager
Prof. Alessandro Alboresi

Staff
Full time: Alessandra Bellan
Part Time: Elisabetta Barizza, Luca Cesarotto, Stefania Marcato, Anna Pavanello, Anna Segalla

Instruments:

• Cell culture and plant cultivation: 9 growth chambers with different illumination / temperature / humidity and light regime.
• Transformation: particle gun from Beambio and electroporator from ECM® 630 Electroporator;
• Phenotyping:  Fluorcam from Photon System Instruments (closed and open), stereomicroscope with camera;
• LI-6800 Photosynthesis System for measurement of Leaf-Level Gas Exchange & Fluorescence
• Felix F-750 for Near Infrared Spectroscopy and measurement of fruit quality.

Activities:

• Design of genome editing approaches (eg. support in guide RNA design)
• Optimization of DNA extraction protocols from plant samples (also critical ones, rich of phenols etc)
• Training to use the instruments available for transformation and phenotyping.
• Management and maintenance of the growth chambers entrusted to the facility.
• Experimental Greenhouse Management.
• Plant growing support.
• Maintenance of solid plant cell cultures, lines of plants, collecting seeds ex Arabidopsis.
• Maintenance of algae / cyanobacteria strains collection.
• Measurement of leaf gas exchange.
• Measurement of fruit quality.