Curriculum in PDF: English CV

Since 2000, I have developed an extensive experience in the analysis of gene expression in model (H. sapiens and M. musculus) and non-model organisms (M. galloprovincialis and E. superba) by the application of new techniques of molecular biology, genetic engineering and genomics. I have developed and established a new technique for the production of subtracted cDNA libraries, in order to facilitate the identification of rare transcripts in human skeletal muscle. During my specialization in Medical genetics, I have applied cDNA and oligonucleotides microarrays to define the gene expression profiles of pediatric tumors: acute lymphoblastic leukemia and alveolar rhabdomyosarcoma. During my PhD, I have been particularly involved in defining microRNA transcriptional signature in breast and melanoma tumors, but also in rhabdomyosarcoma cell lines, in γ-irradiated human peripheral blood lymphocytes and in the atrophic process of skeletal muscle. I have also contributed to the characterization of EsCry, the first clock gene in E. superba, and I have also contributed to the first characterization of the krill diurnal transcriptome under natural conditions. Finally, I was involved in the functional characterization of: i) drim2, the D. melanogaster homolog of the yeast mitochondrial deoxynucleotide transporter that is essential to maintain mitochondrial function. ii) dSurf1 that encodes an inner membrane mitochondrial protein involved in COX assembly and mutations in the SURF1 gene are the most prevalent cause of Leigh Syndrome and iii) dMpv17 that encodes a mitochondrial inner membrane protein which absence or malfunction causes OXPHOS failure and mtDNA depletion.