Leica SP5 Confocal inverted Microscope

SP5 is fitted with these objective (Eyepieces: 10X):

Objective type

Magnification /Aperture

Immersion

HC PL FLUOTAR 2.5X / 0.07

2.5X / 0.07

Air

HC PL FLUOTAR 5X / 0.15

5X / 0.15

Air

HC PL FLUOTAR 10X / 0.30

10X / 0.30

Air

HC PL FLUOTAR 20X / 0.50*

20X / 0.50

Air

HC PL FLUOTAR 40X / 0.70*

40X / 0.70

Air

HC PL FLUOTAR 63X / 0.80*

63X / 0.80

Air

HC PL FLUOTAR 100X / 1.30 OIL*

100X / 1.30

OIL

Note: * insert condenser

Filter cubes

Filter Cube

Excitation Range

Excitation Filter

Dichromatic Mirror

Suppression Filter

Filter position*

A

UV

BP 340-360

400

LP 425

2

N2.1

green

BP 515-560

580

LP 590

3

I3

blue

BP 450-490

510

LP 515

4

Brightfield

-

-

-

-

1

suitable for fluorophores, see

Laser