Leica DMR Fluorescence Microscope

DMR is fitted with these objective (Eyepieces: 10X):

Objective type

Magnification /Aperture

Immersion

HC PL FLUOTAR 2.5X / 0.07

2.5X / 0.07

Air

HC PL FLUOTAR 5X / 0.15

5X / 0.15

Air

HC PL FLUOTAR 10X / 0.30

10X / 0.30

Air

HC PL FLUOTAR 20X / 0.50*

20X / 0.50

Air

HC PL FLUOTAR 40X / 0.70*

40X / 0.70

Air

HC PL FLUOTAR 63X / 0.80*

63X / 0.80

Air

HC PL FLUOTAR 100X / 1.30 OIL*

100X / 1.30

OIL

Note: * insert condenser

Filter cubes

Filter Cube

Excitation Range

Excitation Filter

Dichromatic Mirror

Suppression Filter

Filter position*

A

UV

BP 340-360

400

LP 425

2

N2.1

green

BP 515-560

580

LP 590

3

I3

blue

BP 450-490

510

LP 515

4

Brightfield

-

-

-

-

1

suitable for fluorophores: Correlation of fluorochromes and filter cubes

Instructions:

  1. Switch on the light on the right side (bottom) of the microscope
  2. If you need, switch on the fluorescence module on the right
  3. The basic setting of the DMR from top to bottom is (please, at the end of your session reset the same setting):
    a) incident light polarizer out on the left of the eyepieces.
    b) *fluorescence filter position: 1 (brightfield)
    c) interference filter position: A
    d) Interference filter under the condenser: H (brightfield)
    e) polarized on the base: off
  4. At the end do not forget to close the LAS software
  5. If you are the last user, please switch off all lamps, computer and cover the microscope.