SPOTLIGHT: DiBio Imaging Facility

Pubblicato il: 23.02.2021 10:00

The facility manages advanced bioimaging equipment and provides technical and administrative management, equipment maintenance and upgrades, user training and support, laboratory and promotional activities:

Electron microscopy

The DiBioEM offers the following services:

preparation and observation of samples for conventional transmission electron microscopy - preparation and observation of macromolecules, cell fractions, nanoparticles, plastics and polymers - immunocytochemistry in pre and post embedding - technical and application advices - results interpretation - techniques update -  equipment maintenance and upgrade - teaching activities - users training and tutorials -promotional activities.

Equipment

- TEM FEI Tecnai G2 with OSIS Veleta Camera

- Vitrobot FEI Mark III

- Ultramicrotomes: LKB Ultrotome III, LKB Ultrotome V, Reichert-Jung Ultracut 70170

- Sputter coater/glow discarge Edwards S150B

- Critical point dryer Polaron CDP7501

Optical and confocal microscopy

The different instruments cover a wide range of microscopy applications: analyses of fixed and in vivo samples as well as non-biological specimens employing a thermostated and CO2 chamber, DIC and phase contrast and different fluorescent filters (DAPI, FITC, Cy3, Cy5, Chlor, etc.)

Equipment

- Zeiss LSM 700 confocal microscope

- Leica Sp5 confocal microscope

- Leica MZ16

- Leica DMR

- Leica 5000B

- Leica DMI 4000B

Hight-Throughput Screening Facility

About us:

Since June 2019 the High Throughput Screening (HiTS@UniPD) facility at the Department of Biology, University of Padua, allows to perform medium/high throughput screens as well as small-scale research projects that can benefit from the use of our equipment and expertise in automation, assay development and data acquisition.

HiTS@UniPD MISSION

To provide screening services and access to state-of-the-art high throughput imaging technologies;

To offer the best support if you need to unbiasedly address life science questions.

Equipment and applications

-An Operetta high content Imaging system (Perkin- Elmer);

- Two automated liquid handling platforms;

- Chemical compounds and genome wide libraries;

- Automated nucleic acid purification platform.

Our technology is suitable for any type of sample, from 2D to 3D biological models including cell monolayers, isolated muscle fibers, parasites, moss/plants, spheroids, organoids and whole organisms (e.g. Zebrafish). 

Your applications, your way:

• Fixed cell assays;

• Live cell experiments (as an example, FRET);

• Imaging of 3D cell models;

• Analysis of Complex cell models (e.g. o-culture systems, iPSCs, primary cell cultures);

• Drug discovery screens;

• Zebrafish imaging;

• Quantification of:

-Fluorescence intensity;

-Number of cells;

-Area of nucleus or cells;

-Mean intensity of nuclear and cytoplasm fluorescence;

-Organelles morphology;

-Analysis of cell differentiation, cell proliferation and migration.

Contact us for additional details:

DiBio Imaging Facility

Department of Biology,

Via U. Bassi, 58/B,

35131, Padova (PD) Italy

E-mail: imaging.biologia@unipd.it; hits.biologia@unipd.it;